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| 别名: | CD70,CD27LG,TNFSF7,TNFSF7G,CD27L | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human CD27 Ligand / CD70 Protein, Fc Tag (active trimer) (MALS verified)
CD70,CD27LG,TNFSF7,TNFSF7G,CD27L
Human CD27 Ligand, Fc Tag (active trimer) (MALS verified) is expressed from human 293 cells. It contains AA Ser 52 - Pro 193 trimer Design.
Predicted N-terminus: Pro
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 74.1 kDa. The protein migrates as 130-150 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris, Glycine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human CD27 Ligand, Fc Tag (active trimer) (MALS verified) on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human CD27 Ligand, Fc Tag (active trimer) (MALS verified) is more than 85% in HP-SEC, and the molecular weight of this protein is around 160-190 kDa verified by SEC-MALS.
Report
Immobilized Human CD27, His Tag at 2 μg/mL (100 μL/well) can bind Human CD27 Ligand, Fc Tag (active trimer) (MALS verified) with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
Serial dilutions of Anti-CD27 Ligand (cusatuzumab) Antibody, Human IgG1 were added into Human CD27 Ligand, Fc Tag (active trimer) (MALS verified) : Biotinylated Human CD27, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.74034 μg/mL (Routinely tested).
