PE-Labeled Human EGFRvIII Protein, His Tag (Site-specific conjugation)

PE-Labeled Human EGFRvIII Protein, His Tag (Site-specific conjugation)

分子别名（Synonym）

EGFRvIII

表达区间及表达系统（Source）

PE-Labeled Human EGFRvIII, His Tag s produced via site-specific conjugation of PE to Human EGFRvIII (25-378), His Tag under optimal conditions with a proprietary technology. Human EGFRvIII, His Tagis expressed from human 293 cells . It contains AA Leu 25 - Ser 378

Predicted N-terminus: Leu 25

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 42.2 kDa.

Conjugate

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

活性（Bioactivity）-FACS

5e5 of anti-EGFRvIII CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human EGFRvIII Protein, His Tag  and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

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