Human EGF R Protein, Llama IgG2b Fc Tag, low endotoxin

Human EGF R Protein, Llama IgG2b Fc Tag, low endotoxin

分子别名（Synonym）

EGFR,ERBB,ERBB1,HER1,PIG61,mENA

表达区间及表达系统（Source）

Human EGF R, Llama IgG2b Fc Tag is expressed from human 293 cells . It contains AA Leu 25 - Ser 645 .

Predicted N-terminus: Leu 25

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a llama IgG2b Fc tag at the C-terminus.

The protein has a calculated MW of 96.4 kDa. The protein migrates as 115-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by reduced SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Human EGF R, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

获取HPLC数据

 

活性（Bioactivity）-ELISA

Immobilized Human EGF, His Tag at 5 μg/mL (100 μL/well) can bind Human EGF R, Llama IgG2b Fc Tag with a linear range of 0.2-5 ng/mL (QC tested).

Protocol

Immobilized Human EGF R, Llama IgG2b Fc Tag 

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