Human IL-17A / CTLA-8 Protein, Tag Free (MALS verified)

Human IL-17A / CTLA-8 Protein, Tag Free (MALS verified)

分子别名（Synonym）

IL-17A,Interleukin-17A,CTLA-8,IL-17

表达区间及表达系统（Source）

Human IL17A, Tag Free is expressed from human 293 cells. It contains AA Gly 24 - Ala 155.

Predicted N-terminus: Gly 24

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 15.1 kDa. As a result of glycosylation, the protein migrates as 16 kDa, 17 kDa and 19 kDa under reducing (R) condition, and 27-34 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Human IL17A, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

The purity of Human IL17A, Tag Freeis more than 95% and the molecular weight of this protein is around 30-46 kDa verified by SEC-MALS.

Report

 

活性（Bioactivity）-ELISA

Immobilized Human IL17A, Tag Free at 0.5 μg/mL (100 μL/well) can bind Human IL-17 RA, His Tagwith a linear range of 8-125 ng/mL (QC tested).

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