Human IL-2 R beta / CD122 Protein, His Tag (SPR verified)_细胞生物学_生命科学

Human IL-2 R beta / CD122 Protein, His Tag (SPR verified)

别名:	IL2RB,RP5-1170K4.6,CD122,P70-75
		储存条件:	-20℃

货号	规格	可用库存	销售价(RMB)	您的折扣价(RMB)	购买数量
ZY-CD2-H5221-100ug	100ug	现货	~~3500.00~~	3500

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熔点:
密度:
储存条件:	-20℃

Human IL-2 R beta / CD122 Protein, His Tag (SPR verified)

分子别名（Synonym）

IL2RB,RP5-1170K4.6,CD122,P70-75

表达区间及表达系统（Source）

Human IL-2 R beta, His Tag (SPR verified) is expressed from human 293 cells. It contains AA Ala 27 - Asp 239 ).

Predicted N-terminus: Ala 27

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 25.4 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Human IL-2 R beta, His Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

获取HPLC数据

活性（Bioactivity）-SPR

Human IL-2 R beta, His Tag (SPR verified) captured on CM5 chip via anti-His antibody, can bind Human IL-2, Tag Free with an affinity constant of 0.525 μM as determined in a SPR assay .

Protocol

Human IL-2 R beta, His Tag (SPR verified) captured on CM5 chip via anti-His antibody, can bind Human IL-15, Tag Freewith an affinity constant of 21.7 nM as determined in a SPR assay (Routinely tested).

Protocol

活性（Bioactivity）-BLI

Loaded Human IL-2 R beta, His Tag (SPR verified) on HIS1K Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 0.46 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

活性（Bioactivity）-Cell based assay

Human IL-2 R beta, His Tag (SPR verified)inhibits the IL-15-dependent proliferation of Mo7e cells. The EC50 for this effect is 0.38-0.45 µg/mL

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

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精细化学

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标准品

Human IL-2 R beta / CD122 Protein, His Tag (SPR verified)

分子别名（Synonym）

IL2RB,RP5-1170K4.6,CD122,P70-75

表达区间及表达系统（Source）

Human IL-2 R beta, His Tag (SPR verified) is expressed from human 293 cells. It contains AA Ala 27 - Asp 239 ).

Predicted N-terminus: Ala 27

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 25.4 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Human IL-2 R beta, His Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

获取HPLC数据

活性（Bioactivity）-SPR

活性（Bioactivity）-BLI

Loaded Human IL-2 R beta, His Tag (SPR verified) on HIS1K Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 0.46 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

活性（Bioactivity）-Cell based assay

Human IL-2 R beta, His Tag (SPR verified)inhibits the IL-15-dependent proliferation of Mo7e cells. The EC50 for this effect is 0.38-0.45 µg/mL

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

精细化学

分析色谱

材料科学

生命科学

标准品

Human IL-2 R beta / CD122 Protein, His Tag (SPR verified)

分子别名（Synonym）

IL2RB,RP5-1170K4.6,CD122,P70-75

表达区间及表达系统（Source）

Human IL-2 R beta, His Tag (SPR verified) is expressed from human 293 cells. It contains AA Ala 27 - Asp 239 ). Predicted N-terminus: Ala 27 Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.4 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Human IL-2 R beta, His Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. 获取HPLC数据

活性（Bioactivity）-SPR

活性（Bioactivity）-BLI

Loaded Human IL-2 R beta, His Tag (SPR verified) on HIS1K Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 0.46 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Protocol

活性（Bioactivity）-Cell based assay

Human IL-2 R beta, His Tag (SPR verified)inhibits the IL-15-dependent proliferation of Mo7e cells. The EC50 for this effect is 0.38-0.45 µg/mL

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

Human IL-2 R beta, His Tag (SPR verified) is expressed from human 293 cells. It contains AA Ala 27 - Asp 239 ).

Predicted N-terminus: Ala 27

Request for sequence

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 25.4 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

Human IL-2 R beta, His Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

获取HPLC数据

Loaded Human IL-2 R beta, His Tag (SPR verified) on HIS1K Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 0.46 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol