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Human IL-21 R Protein, Fc Tag (MALS verified)

Human IL-21 R Protein, Fc Tag (MALS verified)
别名: IL21R,CD360,NILR
储存条件: -20℃ 
Human IL-21 R Protein, Fc Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-ILR-H5257-5 200ug 现货 0.00
ZY-ILR-H5257-200ug 200ug 现货 3100.00 3100
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熔点:
密度:
储存条件: -20℃

 

Human IL-21 R Protein, Fc Tag (MALS verified)

分子别名(Synonym)

IL21R,CD360,NILR

表达区间及表达系统(Source)

Human IL-21 R, Fc Tag is expressed from human 293 cells . It contains AA Cys 20 - Pro 236.

Predicted N-terminus: Cys 20

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 51.5 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IL-21 R, Fc Tag (Cat. No. ILR-H5257) SDS-PAGE gel

Human IL-21 R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human IL-21 R, Fc Tag  is more than 90% and the molecular weight of this protein is around 115-135 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL-21, Tag Free at 5 μg/mL (100 μL/well) can bind Human IL-21 R, Fc Tag  with a linear range of 0.006-0.05 μg/mL (QC tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human IL-21, Tag Freeimmobilized on CM5 Chip can bind Human IL-21 R, Fc Tag with an affinity constant of 0.412 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

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