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| 别名: | Latent TGF-beta 1,Latent TGFB1,TGFB1,CED,DPD1,LAP,TGF-beta-1,TGFB | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 密度: | |
| 储存条件: | -20℃ |
Cynomolgus Latent TGF-beta 1 (C33S) Protein, His Tag (HPLC verified)
Latent TGF-beta 1,Latent TGFB1,TGFB1,CED,DPD1,LAP,TGF-beta-1,TGFB
Cynomolgus Latent TGF-Beta 1 (C33S), His Tagis expressed from human 293 cells. It contains AA Leu 30 - Ser 390 (C33S) .
Predicted N-terminus: His & Leu 280
Request for sequence
This protein carries a polyhistidine tag at the N-terminus. The precursor proprotein is partially cleaved by FURIN to form mature Transforming growth factor beta-1 (TGF-beta-1) and Latency-associated peptide (LAP) chains with calculated MW of 12.7 kDa and 30.4 kDa respectively. The protein migrates as 50-60 kDa (precursor proprotein), 38-45 kDa (LAP) and 13 kDa (TGFB1) under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Cynomolgus Latent TGF-Beta 1 (C33S), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Cynomolgus Latent TGF-Beta 1 (C33S), His Tag was greater than 90% as determined by SEC-HPLC.
Immobilized Cynomolgus Latent TGF-Beta 1 (C33S), His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free with a linear range of 1-31 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus Latent TGF-Beta 1 (C33S), His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB8 Heterodimer Protein, His,Avitag&Tag Free ith a linear range of 5-78 ng/mL (Routinely tested).
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
