Anti-YKL-40/CHI3L1 antibody [A3G10]

Anti-YKL-40/CHI3L1 antibody [A3G10]

• 概述

• 产品描述Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.
• 产品名称Anti-YKL-40/CHI3L1 antibody [A3G10]
• 分子量43 kDa
• 种属反应性Human
• 验证应用WB,IHC-P
• 抗体类型小鼠单抗
• 免疫原Recombinant protein within extracellular domain of human CHI3L1.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein G affinity purified.
• 亚细胞定位Extracellular space, Endoplasmic reticulum, Cytoplasm.
• 其它名称
  • 39 kDa synovial protein antibody
  • ASRT7 antibody
  • Cartilage glycoprotein 39 antibody
  more

• 应用

  WB:1:500-1:2,000
  IHC-P:1:50-1:200

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  Fig1: Western blot analysis of YKL-40/CHI3L1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-YKL-40/CHI3L1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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