Anti-Cdk4 antibody [5-C3-G3]

Anti-Cdk4 antibody [5-C3-G3]

• 概述

• 产品描述Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression . Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-PCTAIRE-3, PITALRE and PITSLRE. Cdk4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. This association with a D-type cyclin upregulates Cdk4 activity, whereas binding to the Cdk inhibitor p16 downregulates Cdk4 activity. Activation of the Cdk4-cyclin complexes requires phosphorylation on a single threonyl residue of Cdk4, catalyzed by a Cdk-activating protein (CAK).
• 产品名称Anti-Cdk4 antibody [5-C3-G3]
• 分子量34kDa
• 种属反应性Human
• 验证应用WB, IHC-P
• 抗体类型小鼠单抗
• 免疫原This antibody is produced by immunizing mice with recombinant protein corresponding to a region of Cdk4 (aa 80-320).
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2c
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Nucleus. Membrane.
• 其它名称
  • Cdk 4 antibody
  • cdk4 antibody
  • CDK4 protein antibody
  more

• 应用

  WB: 1:500-1:1,000
  IHC-P: 1:50-1:200

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  Fig1: Western blot analysis of Cdk4 on Hela using anti-Cdk4 antibody at 1/1,000 dilution.
  
  
  
   

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Cdk4 antibody. Counter stained with hematoxylin.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Cdk4 antibody. Counter stained with hematoxylin.

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