Anti-Hint1 antibody [4G12]

Anti-Hint1 antibody [4G12]

• 概述

• 产品描述Hint1 (histidine triad nucleotide binding protein 1), also known as PRKCNH1 or PKCI1, is a 126 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one HIT (histidine triad) domain. Widely expressed and existing as a homodimer, Hint1 functions to hydrolyze adenosine 5'-monophosphoramidate substrates, such as AMP-N-alanine methyl ester, AMP-morpholidate and AMP-α-acetyl lysine methyl ester, and may also inhibit PKC function and influence apoptosis. Knockdown of Hint1 mRNA results in downregulation of p53 and Bax, suggesting that Hint1 is a haploinsufficient tumor suppressor. Also, it is suspected that promoter hypermethylation of the gene encoding Hint1 may play a role in hepatocarcinogenesis.
• 产品名称Anti-Hint1 antibody [4G12]
• 分子量14kDa
• 种属反应性Human
• 验证应用WB,IP,IF
• 抗体类型小鼠单抗
• 免疫原Amino acids 8-47 mapping near the N-terminus of Hint 1 of human origin.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm, Nucleus
• 其它名称
  • Adenosine 5' monophosphoramidase antibody
  • Adenosine 5'-monophosphoramidase antibody
  • HINT 1 antibody
  more

• 应用

  WB: 1:100-1:1,000
  IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate).
  IF: 1:50-1:500

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  Fig1: Western blot analysis of Hint1 expression in Jurkat (A) and HepG2 (B) whole cell lysates.

   

  

  Fig2: Immunofluorescencestaining of methanol-fixed HeLa cells showing cytoplasmic localization.

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