Anti-P2RX7 antibody [G1-D6]

Anti-P2RX7 antibody [G1-D6]

• 概述

• 产品描述The P2X receptor family is comprised of ligand-gated ion channels that allow for the increased permeability of calcium into the cell in response to extracellular ATP. The seven P2X receptors, P2X1-P2X7, form either homomeric or heteromeric channels or both. They are characterized by intracellular amino- and carboxy-termini. P2X receptors are expressed in a wide variety of tissues, including neurons, prostate, bladder, pancreas, colon, testis and ovary. The major function of the P2X receptors is to mediate synaptic transmissions between neurons and to other tissues via the binding of extracellular ATP, which acts as a neurotransmitter. The P2X receptors may be involved in the onset of necrosis or apoptosis after prolonged exposure to high concentrations of extracellular ATP.
• 产品名称Anti-P2RX7 antibody [G1-D6]
• 分子量70 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Cell membrane.
• 其它名称
  • ATP receptor antibody
  • P2rx7 antibody
  • P2RX7_HUMAN antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of P2RX7 on human P2RX7 recombinant protein using anti-P2RX7 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of P2RX7 on HEK293 (1) and P2RX7-hIgGFc transfected HEK293 (2) cell lysate using anti-P2RX7 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of P2RX7 on different cell lysate using anti-P2RX7 antibody at 1/1,000 dilution.
  Positive control: Line1: A431 Line2: U251 Line3: Hela Line4: U937 Line5: HepG2

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-P2RX7 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of Hela cells with P2RX7 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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