Anti-KDM4A antibody [E7-H1]

Anti-KDM4A antibody [E7-H1]

• 概述

• 产品描述JMJD2A (jumonji domain containing 2A), also designated jumonji C domain-containing histone demethylation protein 3A, is a 1,064 amino acid protein encoded by the human gene JMJD2A. JMJD2A belongs to the JHDM3 histone demethylase family and contains one JmjC domain, one JmjN domain, two PHD-type zinc fingers and two Tudor domains. JMJD2A is histone de-methylase that specifically demethylates Lys 9 and Lys 36 residues of Histone H3, thereby playing a central role in histone code. It does not demethylate histone H3 Lys 4, H3 Lys 27 nor H4 Lys 20, however, it will demethylate tri-methylated H3 Lys 9 and H3 Lys 36 residue, while it has no activity on mono- and dimethylated residues. JMJD2A demethylation of lysine residues will generate formaldehyde and succinate. It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. JMJD2A is a ubiquitously expressed nuclear protein.
• 产品名称Anti-KDM4A antibody [E7-H1]
• 分子量120 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • JHDM3A antibody
  • JmjC domain containing histone demethylation protein 3A antibody
  • JmjC domain-containing histone demethylation protein 3A antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:100-1:500
  IHC-P: 1:50-1:200

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  Fig1: Western blot analysis of KDM4A on HEK293 (1) and KDM4A-hIgGFc transfected HEK293 (2) cell lysate using anti-KDM4A antibody at 1/1,000 dilution.

   

  

  Fig2: ICC staining KDM4A (green) and actin filaments (red) in NTERA-2 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human larynx cancer tissue using anti-KDM4A antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-KDM4A antibody. Counter stained with hematoxylin.

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