Anti-BAFF antibody [A2-C3]

Anti-BAFF antibody [A2-C3]

• 概述

• 产品描述TNF cytokine family member TALL-1 (also designated BAFF, TNFSF13B and BLyS), is a type II membrane protein that shares characteristics with other members of the tumor necrosis factor (TNF) cytokine family. TALL-1 is expressed abun-dantly in peripheral blood leukocytes, monocytes and macrophages. TALL-1 is critically involved in the regulation of infections, inflammation, autoimmune diseases and tissue homeostasis. TALL-1 is a potent B cell stimulatory factor which is functionally involved in B cell proliferation and may be a primary mediator in B cell-associated autoimmune diseases. The C-terminal extracellular domain has b jelly roll topography and is important in ligand trimerization. The binding of the ligand to its respective receptor induces oligomerization, initiating downstream signaling events. Intrinsic to oligomerization is the formation of the receptor binding site at the junction between neighboring subunits, creating a multivalent ligand.
• 产品名称Anti-BAFF antibody [A2-C3]
• 分子量31 kDa
• 种属反应性Human
• 验证应用WB,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Secreted and Cell membrane.
• 其它名称
  • B cell activating factor receptor antibody
  • B-cell-activating factor receptor antibody
  • BAFF R antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of BAFF on human BAFF recombinant protein using anti- BAFF antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of BAFF on HEK293 (1) and BAFF -hIgGFc transfected HEK293 (2) cell lysate using anti- BAFF antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of BAFF on SK-N-SH (1) and MOLT4 (2) cell lysate using anti- BAFF antibody at 1/1,000 dilution.

   

  

  Fig4: ICC staining BAFF (green) and Actin filaments (red) in SMMC-7721 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Flow cytometric analysis of Hela cells with BAFF antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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