Anti-DDX1 antibody [B2-E5]

Anti-DDX1 antibody [B2-E5]

• 概述

• 产品描述DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX1 mRNA has a widespread distribution in human fetal tissue, but is not uniformly expressed in all tissues. Chicken DDX1, which shares 93% identity with human DDX1, shows highest levels of expression during the early stages of development. Tissue maturation typically correlates with a decrease in DDX1 expression, although DDX1 levels remain elevated in late embryonic retina and brain.
• 产品名称Anti-DDX1 antibody [B2-E5]
• 分子量82 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus. Cytoplasm. Cytoplasmic granule.
• 数
• 其它名称
  • ATP dependent helicase DDX1 antibody
  • ATP-dependent RNA helicase DDX1 antibody
  • DBP RB antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:200-1:500
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of DDX1 on human DDX1 recombinant protein using anti-DDX1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of DDX1 on HEK293 (1) and DDX1-hIgGFc transfected HEK293 (2) cell lysate using anti-DDX1 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of DDX1 on different cell lysate using anti-DDX1 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Hela
  Lane 2: MCF-7
  Lane 3: A431
  Lane 4: PC-3
  Lane 5: Jurkat

   

  

  Fig4: ICC staining DDX1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-DDX1 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-DDX1 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with DDX1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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