Anti-CDH11 antibody [A6-C12]

Anti-CDH11 antibody [A6-C12]

• 概述

• 产品描述Cadherins are calcium dependent cell adhesion proteins, a family of Ca++-dependent adhesion molecules that influence cell-cell binding and are critical to the maintenance of tissue structure and morphogenesis. OB-cadherin (osteoblast-cadherin, cadherin-11, OSF-4) has two forms, OB-cadherin-1 and OB-cadherin-2. OB-cadherin-2 has a truncated cytoplasmic domain, missing amino acids 694-796. Both OB-cadherins are expressed in osteoblastic cell lines with low expression seen in lungs, testis and brain. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types
• 产品名称Anti-CDH11 antibody [A6-C12]
• 分子量88 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cell membrane
• 其它名称
  • CAD11 antibody
  • CAD11_HUMAN antibody
  • Cadherin 11 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

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  Fig1: Western blot analysis of CDH11 on human CDH11 recombinant protein using anti-CDH11 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CDH11 on HEK293 (1) and CDH11-hIgGFc transfected HEK293 (2) cell lysate using anti-CDH11 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of CDH11 on MCF-7 (1), Jurkat (2) and HEK293 (3) cell lysate using anti-CDH11 antibody at 1/1,000 dilution.

   

  

  Fig4: ICC staining CDH11 (green) and Actin filaments (red) in HL-7702 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CDH11 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Hela cells with CDH11 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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