Anti-CD6 antibody [12-D1]

Anti-CD6 antibody [12-D1]

• 概述

• 产品描述CD6 is a type I transmembrane glycoprotein that is present on mature thymocytes, peripheral T cells and a subset of B cells. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T-cell activation and the size difference between the CD6 forms is due in part to differences in phosphorylation state. CD6 protein contains a 24-amino acid signal sequence, three extracellular "scavenger receptor cysteine-rich" (SRCR) domains, a membrane-spanning domain, and a 44-amino acid cytoplasmic domain. CD6 shows significant homology to CD5. CD6, which is also found in brain and B cell chronic lymphocytic leukemias, plays an important role in interactions of thymocytes with thymic epithelial cells. CD6 molecules can physically associate with the TCR/CD3 complex.
• 产品名称Anti-CD6 antibody [12-D1]
• 分子量72 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Membrane.
• 其它名称
  • CD_antigen=CD6 antibody
  • CD6 antibody
  • CD6 antigen Tp120 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:200-1:500
  IHC-P: 1:200-1:500

•  

  

  Fig1: Western blot analysis of CD6 on human CD6 recombinant protein using anti-CD6 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CD6 on HEK293 (1) and CD6-hIgGFc transfected HEK293 (2) cell lysate using anti-CD6 antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining CD6 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CD6 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-CD6 antibody. Counter stained with hematoxylin.

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