Anti-FBXL10 antibody [6G-11]

Anti-FBXL10 antibody [6G-11]

• 概述

• 产品描述FBXL10 (F-box and leucine-rich repeat protein 10), also known as Fbl10, CXXC2 (CXXC-type zinc finger protein 2), PCCX2, KDM2B, JEMMA (jumonji domain, EMSY-interactor, methyltransferase motif protein) or JHDM1B (jumonji C domain-containing histone demethylase 1B), is a nuclear protein that contains one F-box domain, a CXXC-type zinc finger, one JMJC domain, four leucine-rich repeats and one PHD-type zinc finger. FBXL10 belongs to the Fbls class of F-box proteins that contain leucine-rich repeats in addition to their F-box motif. F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. In addition to its role in substrate recognition as a component of the E3 complex, FBXL10 functions as a dimethylation-specific demethylase, binding iron as a cofactor and demethylating lysine-36 of Histone H3. This suggests that FBXL10 plays a central role in the histone code.
• 产品名称Anti-FBXL10 antibody [6G-11]
• 分子量152 kDa
• 种属反应性Human
• 验证应用WB,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • [Histone-H3]-lysine-36 demethylase 1B antibody
  • CXXC-type zinc finger protein 2 antibody
  • CXXC2 antibody
  more

• 应用

  WB: 1:500-1:1,000
  FC: 1:50-1:200

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  Fig1: Western blot analysis of FBXL10 on human FBXL10 recombinant protein using anti-FBXL10 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of FBXL10 on HEK293 (1) and FBXL10-hIgGFc transfected HEK293 (2) cell lysate using anti-FBXL10 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of FBXL10 on A549 cell lysate using anti-FBXL10 antibody at 1/1,000 dilution.

   

  

  Fig4: Flow cytometric analysis of Hela cells with FBXL10 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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