Anti-AQP2 antibody [7H8B5]

Anti-AQP2 antibody [7H8B5]

• 概述

• 产品描述AQP2 is found in the apical cell membranes of the kidney's collecting duct principal cells and in intracellular vesicles located throughout the cell. It is the only aquaporin regulated by vasopressin.[5] The basic job of aquaporin 2 is to reabsorb water from the urine while its being removed from the blood by the kidney. Aquaporin 2 is in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed, vasopressin binds to the cell surface vasopressin receptor thereby activating a signaling pathway that causes the aquaporin 2 containing vesicles to fuse with the plasma membrane, so the aquaporin 2 can be used by the cell. Mutations in this channel are associated with nephrogenic diabetes insipidus, which can be autosomal dominant or recessive. Mutations in the vasopressin receptor cause a similar X-linked phenotype. Lithium, which is often used to treat bipolar disorder, can cause acquired diabetes insipidus (characterized by the excretion of large volumes of dilute urine) by decreasing the expression of the AQP2 gene. The expression of the AQP2 gene is increased during conditions associated with water retention such as pregnancy and congestive heart failure.
• 产品名称Anti-AQP2 antibody [7H8B5]
• 分子量28.8kDa
• 种属反应性Human
• 验证应用WB
• 抗体类型小鼠单抗
• 免疫原Purified recombinant fragment of human AQP2 (AA: 149-271) expressed in E. Coli.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS with 0.05% sodium azide.
• 亚型IgG1
• 纯化方式Protein G purified.
• 亚细胞定位Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical
• 其它名称
  • ADH water channel antibody
  • AQP 2 antibody
  • AQP CD antibody
  more

• 应用

  WB: 1:500-1:2,000

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  Fig1: Western blot analysis of AQP2 against human AQP2 (AA: 149-271) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig2: Western blot analysis of AQP2 against HEK293 (1) and AQP2 (AA: 149-271)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig3: Western blot analysis of AQP2 against K562 (1), HeLa (2), HCT116 (3), and SW480 (4) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (E was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

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