Anti-RIM 2 antibody [3G2]

Anti-RIM 2 antibody [3G2]

• 概述

• 产品描述Rab3, a neural/neuroendocrine-specific member of the Rab family, is involved in Ca2+-regulated exocytosis. Rab3 functions in an inhibitory capacity by controlling the recruitment of secretory vesicles into a releasable pool at the plasma membrane. Rim (rab3 interacting molecule), a putative effector protein for Rab3s, is composed of an N-terminal zinc-finger motif and C-terminal PDZ and C2 domains. Rim exists as two variants, Rim1 and Rim2, produced by alternative splicing. The 3’-end of the Rim2 gene produces an independent mRNA that encodes a smaller protein referred to as Nim2, which like Rim, also regulates exocytosis. Rim serves as a Rab3-dependent regulator of synaptic-vesicle fusion by forming a GTP-dependent complex between synaptic plasma membranes and docked synaptic vesicles. Both Rim1 and Rim2 can bind to cAMP-GEFII, which is a direct target of cAMP in regulated exocytosis and is responsible for cAMP-dependent, PKA-dependent exocytosis. Rim also localizes on the plasma membrane of INS-1E cells and pancreatic beta-cells. Rab3 binding domain of Rim enhances glucose-stimulated secretion in intact cells and Ca2+-stimulated exocytosis in permeabilized cells, suggesting that Rim may also play a regulatory role in insulin secretion.
• 产品名称Anti-RIM 2 antibody [3G2]
• 分子量160kDa
• 种属反应性Human,Rat
• 验证应用WB,IP
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cell membrane, Cell junction
• 其它名称
  • KIAA0751 antibody
  • Non small cell lung cancer RimL3a protein antibody
  • Non small cell lung cancer RimL3c protein antibody
  more

• 应用

  WB: 1:100-1:1,000
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
  IF: 1:50-500
  IHC-P: 1:50-500

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  Fig1: Western blot analysis of Rim2 expression in PC-12 whole cell lysate.

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