Anti-P53 antibody [C8-A11]

Anti-P53 antibody [C8-A11]

• 概述

• 产品描述Cellular tumor antigen p53 is crucial in multicellular organisms, where it regulates the cell cycle and, thus, functions as a tumor suppressor, preventing cancer. As such, p53 has been described as "the guardian of the genome" because of its role in conserving stability by preventing genome mutation. Activated p53 binds DNA and activates expression of several genes including microRNA miR-34a, WAF1/CIP1 encoding for p21 and hundreds of other down-stream genes. p21 (WAF1) binds to the G1-S/CDK (CDK2) and S/CDK complexes (molecules important for the G1/S transition in the cell cycle) inhibiting their activity. p53 expression can be stimulated by UV light, which also causes DNA damage. In this case, p53 can initiate events leading to tanning.
• 产品名称Anti-P53 antibody [C8-A11]
• 分子量~53kDa
• 种属反应性Human,Mouse
• 验证应用WB,ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein G purified.
• 亚细胞定位Cytoplasm, nucleus
• 其它名称
  • Antigen NY-CO-13 antibody
  • BCC7 antibody
  • Cellular tumor antigen p53 antibody
  more

• 应用

  ICC: 1:500- 1:1,000
  IHC-P: 1:200
  WB: 1:500

•  

  

  Fig1: Western blot analysis on 293 cell lysates using anti- p53 mouse mAb.

   

  

  Fig2: ICC staining p53 in A431 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti- p53 mouse monoclonal antibody.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！