Anti-Smad2/3 antibody [4D3]

Anti-Smad2/3 antibody [4D3]

• 概述

• 产品描述Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members.Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.
• 产品名称Anti-Smad2/3 antibody [4D3]
• 分子量55-60kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IP,IF,IHC-P
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm, Nucleus
• 其它名称
  • hMAD 2 antibody
  • hMAD 3 antibody
  • hSMAD2 antibody
  more

• 应用

  WB: 1:1,000
  IP: 1-2 μg per 100-500 μg of total protein
  IF: 1:50-500
  IHC-P: 1:50-500

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  Fig1: Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid tissue showing nuclear staining of glandular cells.

   

  

  Fig2: Western blot analysis of Smad2/3 expression in K-562 (A), HEL 92.1.7 (B), PC-12 (C), WEHI-231 (D) and NIH/3T3 (E) whole cell lysates.

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