Anti-Phosphoserine antibody [5B12]

Anti-Phosphoserine antibody [5B12]

• 概述

• 产品描述Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
• 产品名称Anti-Phosphoserine antibody [5B12]
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P
• 抗体类型小鼠单抗
• 免疫原Purified Protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
• 亚型IgG1
• 纯化方式The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
• 其它名称
  • phospho-Ser antibody
  • pS antibody
  • pSER antibody

• 应用

  WB:1:500-1:2,000
  IHC-P:1:50-1:200

•  

  

  Fig1: Western blot analysis of Phosphoserine on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: Hela cell lysate
  Lane 2: Rat brain lysate
  Lane 3: Mouse brain lysate

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded Human Lung Caricnoma tissue using anti-Phosphoserine antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma tissue using anti-Phosphoserine antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！