Anti-Phospho-CaMKII (Thr 286) antibody [2G1]

Anti-Phospho-CaMKII (Thr 286) antibody [2G1]

• 概述

• 产品描述The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/ threonine protein kinase that is activated by Ca2+ and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes, designated α, β,γandδ, which may or may not be coexpressed in the same tissue types. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory threonine residue at position 177.
• 产品名称Anti-Phospho-CaMKII (Thr 286) antibody [2G1]
• 分子量50kDa
• 种属反应性Rat
• 验证应用WB,IP,IF
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位--
• 其它名称
  • Calcium/calmodulin dependent protein kinase II alpha antibody
  • Calcium/calmodulin dependent protein kinase II beta antibody
  • Calcium/calmodulin dependent protein kinase II delta antibody
  more

• 应用

  WB: 1:100-1,000
  IP: 1-2 μg per 100-500 μg of total protein (1ml of cell lysate)
  IF: 1:50-1:500
  IHC-P: 1:50-1:500

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  Fig1: Western blot analysis of p-CaMKII phosphorylation in untreated (A) and lambda phosphatase treated (B) rat brain tissue extract.

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