Anti-PPARA antibody
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概述
- 产品描述Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors that can be activated by a variety of compounds including fibratus, thiazolidinediones, prostaglandins and fatty acids. Three PPAR subtypes, designated PPARα, PPARβ (also designated PPARδ) and PPARγ, have been described. PPARs promote transcription by forming heterodimers with members of the retinoid X receptor (RXR) family of steroid receptors and binding to specific DNA motifs termed PPAR-response elements (PPREs). PPARα is abundant in primary hepatocytes where it regulates the expression of proteins involved in fatty acid metabolism. Interestingly, both the orphan nuclear hormone receptor LXRα and thyroid receptor (TR) have been shown to act as antagonists of PPARα/RXRα binding to PPREs.
- 产品名称Anti-PPARA antibody
- 分子量52 kDa
- 种属反应性Human,Monkey
- 验证应用WB,FC
- 抗体类型小鼠单抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG1
- 纯化方式Protein A purified.
- 亚细胞定位Nucleus.
- 其它名称
- hPPAR antibody
- MGC2237 antibody
- MGC2452 antibody
more
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应用
WB: 1:500-1:2,000
FC: 1:100-1:200
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Fig1: Western blot analysis of PPARA on human PPARA recombinant protein using anti-PPARA antibody at 1/1,000 dilution.
Fig2: Western blot analysis of PPARA on HEK293 (1) and PPARA-hIgGFc transfected HEK293 (2) cell lysate using anti-PPARA antibody at 1/1,000 dilution.
Fig3: Western blot analysis of PPARA on different cell lysate using anti-PPARA antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: HEK293
Lane 3: COS7
Lane 4: HepG2
Lane 5: Jurkat
Fig4: Flow cytometric analysis of Jurkat cells with PPARA antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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