Anti-PAK3 antibody [F11-G8]

Anti-PAK3 antibody [F11-G8]

• 概述

• 产品描述Three isoforms of serine/threonine kinases, designated aPAK p68, bPAK p65 and gPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The a, b and gPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is Mek kinase, an upstream effector of Mek4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho.
• 产品名称Anti-PAK3 antibody [F11-G8]
• 分子量62 kDa
• 种属反应性Human,Monkey
• 验证应用WB
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm.
• 其它名称
  • Beta PAK antibody
  • bPAK antibody
  • CDKN1A antibody
  more

• 应用

  WB: 1:500

•  

  

  Fig1: Western blot analysis of PAK3 on human PAK3 recombinant protein using anti-PAK3 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PAK3 on HEK293 (1) and PAK3-hIgGFc transfected HEK293 (2) cell lysate using anti-PAK3 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of PAK3 on different cell lysate using anti-PAK3 antibody at 1/1,000 dilution.
  Positive control: Line1: Hela Line2: SK-N-SH Line3: T47D Line4: COS7 Line5: HepG2

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！