Anti-BMP4 antibody [1C-17]

Anti-BMP4 antibody [1C-17]

• 概述

• 产品描述Tumor growth factor, or TGFβ, is the prototypic member of a family of secreted proteins that regulate cellular proliferation and differentiation. Related proteins include the activins and the bone morphogenic proteins or BMPs. Like TGFβ, the BMPs signal through a heteromeric receptor complex (TGFβ R) composed of type I (TGFβ RI) and type II (TGFβ RII) receptors. Both the type I and the type II receptors contain an intrinsic serine/threonine kinase activity. Although signaling downstream of the TGF β R is poorly understood, several proteins have been implicated. Six TGFβ/BMP effector proteins, designated Smad1-6, may function as tumor suppressors. Smad proteins have been suggested to be transcription factors, acting similarly to the Stat family which associates directly with activated receptors and then translocates to the nucleus. Evidence supporting this assertion is drawn from the observation that Smad3 physically associates with the TGFβ R complex and that Smad1 is translocated to the nucleus 30-60 minutes after the addition of BMP-4.
• 产品名称Anti-BMP4 antibody [1C-17]
• 分子量47 kDa
• 种属反应性Human,Rat
• 验证应用WB,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Secreted, extracellular space, extracellular matrix.
• 其它名称
  • zgc:100779 antibody
  • BMP 2B antibody
  • BMP 4 antibody
  more

• 应用

  WB: 1:200-1:1,000
  ICC: 1:50-1:200
  FC: 1:50-1:200

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  Fig1: Western blot analysis of BMP4 on human BMP4 recombinant protein using anti-BMP4 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of BMP4 on HEK293 (1) and BMP4-hIgGFc transfected HEK293 (2) cell lysate using anti-BMP4 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of BMP4 on A549 (1), HepG2 (2), and C6 (3) cell lysate using anti-BMP4 antibody at 1/1,000 dilution.

   

  

  Fig4: ICC staining BMP4 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Flow cytometric analysis of Hela cells with BMP4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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