Anti-B-Raf antibody [F1-F4-E10]

Anti-B-Raf antibody [F1-F4-E10]

• 概述

• 产品描述Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.
• 产品名称Anti-B-Raf antibody [F1-F4-E10]
• 分子量84 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P,ICC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus. Cytoplasm. Cell membrane.
• 其它名称
  • FLJ95109 antibody
  • 94 kDa B raf protein antibody
  • B raf 1 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:100-1:500
  IHC-P: 1:100-1:500

•  

  

  Fig1: Western blot analysis of B Raf on SiHa cell lysate using anti-B Raf antibody at 1/1,000 dilution.

   

  

  Fig2: ICC staining B Raf (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining B Raf (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-B Raf antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-B Raf antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-B Raf antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-B Raf antibody. Counter stained with hematoxylin.

   

  

  Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-B Raf antibody. Counter stained with hematoxylin.

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