Anti-KLHL22 antibody [A6-B10]

Anti-KLHL22 antibody [A6-B10]

• 概述

• 产品描述Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation.
• 产品名称Anti-KLHL22 antibody [A6-B10]
• 分子量72 kDa
• 种属反应性Human,Mouse
• 验证应用WB,ICC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Cytoskeleton.
• 其它名称
  • 2610318I18Rik antibody
  • kelch-like 22 antibody
  • kelch-like family member 22 antibody
  more

• 应用

  WB: 1:500
  ICC: 1:100-1:500

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  Fig1: Western blot analysis of KLHL22 on mouse brain tissue lysate using anti-KLHL22 antibody at 1/1,000 dilution.

   

  

  Fig2: ICC staining KLHL22 (green) and actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

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