Anti-CSK antibody [F1-F3]

Anti-CSK antibody [F1-F3]

• 概述

• 产品描述All members of the Src gene family of tyrosine kinases are characterized by a carboxy terminal domain tyrosine which is highly phosphorylated in the inactive form of the enzyme and phosphorylated to a much lesser extent when the enzyme is active. In the case of Src p60, Y527 is this tyrosine; however, a mutant form of c-Src in which Y527 is replaced by phenylalanine is transforming and displays 5- to 10-fold elevated kinase activity compared to its normal counterpart. Csk has been identified as a Src-related tyrosine kinase having both SH2 and SH3 domains and a catalytic domain but lacking sequences amino terminal to the SH3 domain as well as carboxy terminal regulatory sequences. Csk phosphorylates Src on Y527 and also downregulates Lyn, Fyn and Lck by tyrosine phosphorylation of carboxy terminal regulatory sites.
• 产品名称Anti-CSK antibody [F1-F3]
• 分子量51 kDa
• 种属反应性Human,Mouse,Rat,Monkey
• 验证应用WB,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Cell membrane.
• 其它名称
  • C SRC antibody
  • C SRC kinase antibody
  • C src Tyrosine Kinase antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of CSK on different cell lysates using anti-CSK antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: NIH/3T3
  Lane 2: Hela
  Lane 3: COS7
  Lane 4: Jurkat
  Lane 5: Raw246.7
  Lane 6: A549
  Lane 7: HL-60
  Lane 8: PC-12

   

  

  Fig2: ICC staining CSK (green) and actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Flow cytometric analysis of HL-60 cells with CSK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

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