Anti-p50 dynamitin antibody

Anti-p50 dynamitin antibody

• 概述

• 产品描述This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 23 subunits (11 individual proteins ranging in size from 22 to 150 kD). The subunit is commonly referred to as p50 or dynamitin. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that bind to two copies of p150-glued (DCTN1) and two copies of p24 (DCTN3) to form the dynactin shoulder domain.
• 产品名称Anti-p50 dynamitin antibody
• 分子量Predicted band size: 44 kDa.
• 种属反应性Human,Mouse,Rat
• 验证应用WB,ICC,IHC,FC
• 抗体类型兔多抗
• 免疫原Recombinant protein within human p50 dynamitin aa 1-200.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Protein affinity purified.
• 亚细胞定位Cytoplasm, Cytoskeleton, Dynein, Membrane, Microtubule.
• 其它名称
  • 50 kD dynein associated polypeptide antibody
  • 50 kDa dynein associated polypeptide antibody
  • 50 kDa dynein-associated polypeptide antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:50-1:100
  IHC: 1:200-1:1,000
  FC: 1:50-1:100

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  Fig1: Western blot analysis of p50 dynamitin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibod was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: SHSY5Y cell lysate
  Lane 2: Mouse brain tissue lysate

   

  

  Fig2: ICC staining of p50 dynamitin in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibodfor 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-p50 dynamitin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

   

  

  Fig4: Flow cytometric analysis of p50 dynamitin was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody ( (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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