Anti-GFP antibody

Anti-GFP antibody

• 概述

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  • 产品描述Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.
  • 产品名称Anti-GFP antibody
  • 分子量26 kDa
  • 验证应用WB, ICC, ELISA, IP, FC
  • 抗体类型小鼠单抗
  • 免疫原recombinant protein
  • 偶联Non-conjugated

  性能

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  • 形态Liquid
  • 浓度2 mg/mL.
  • 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • 亚型IgG1
  • 纯化方式Protein A purified.
  • 其它名称
    • GFP antibody
    • Green fluorescent protein antibody
    • yfp antibody

  应用

  • WB: 1:2,000
    ICC: 1:200

   

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    Fig1: Western blot analysis of GFP on transfected K562 with GFP using anti-GFP antibody at 1/2000 dilution.

   

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    Fig2: ICC staining GFP in transfected K562 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

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