Anti-BAX antibody
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概述
- 产品描述BAX is a member of the Bcl-2 gene family. Apoptosis regulator BAX promotes apoptosis by binding to and antagonizing the Bcl-2 protein. In healthy mammalian cells, the majority of BAX is found in the cytosol, but upon initiation of apoptotic signaling, Bax undergoes a conformational shift. Upon induction of apoptosis, BAX becomes organelle membrane-associated, and in particular, mitochondrial membrane associated. The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis. The p53 protein is a transcription factor that, when activated as part of the cell's response to stress, regulates many downstream target genes, including BAX.
- 产品名称Anti-BAX antibody [3-D2]
- 分子量21 kDa
- 种属反应性Human,Mouse
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型小鼠单抗
- 免疫原This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to N-terminal BAX.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgA
- 纯化方式Protein A purified.
- 亚细胞定位Mitochondrion membrane, cytoplas
- 其它名称
- Apoptosis regulator BAX antibody
- BAX antibody
- Bax-protein antibody
more
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应用
WB: 1:500
ICC: 1:200
IHC-P: 1:200
FC: 1:50
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Fig1: Western blot analysis of Bax on different lysates using anti-BAX antibody at 1/500 dilution.
Positive control:
Lane 1: Raji
Lane 2: Mouse lymphoid tissue
Fig2: ICC staining BAX in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using BAX antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using BAX antibody. Counter stained with hematoxylin.
Fig5: Flow cytometric analysis of Hela cells with vimentin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgA (FITC) was used as the secondary antibody.
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