Anti-SOX2 antibody
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概述
- 产品描述SRY (sex determining region Y)-box 2, also known as SOX2, is a transcription factor that is essential to maintain self-renewal of undifferentiated embryonic stem cells. Sox2, Oct-4 and Nanog co-operate in this network, and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency. POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells
- 产品名称Anti-SOX2 antibody
- 分子量33kDa
- 种属反应性Human,Mouse
- 验证应用WB, ICC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within N-terminal human SOX2.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified
- 亚细胞定位Nucleus.
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应用
WB: 1:500-1:1,000
ICC: 1:20-1:50
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Fig1: Western blot analysis on F9 (A) and MCF-7(B) cell lysates using anti-SOX2 rabbit polyclonal antibody.
Fig2: ICC image of SOX2 antibody stained human F9 cells (A) and NCCIT cells (B). The secondary antibody (green) was goat anti-rabbit IgG (H+L) FITC conjugated.
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