Anti-Cyclin B1 antibody
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概述
- 产品描述Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M (mitosis) transition. It interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Four cyclin B1 phosphorylation sites (Ser126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis.
- 产品名称Anti-Cyclin B1 antibody
- 分子量~58kDa
- 种属反应性Human,Mouse
- 验证应用WB, ICC, IHC-P, FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within C-terminal human Cyclin B1.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified
- 亚细胞定位Cytoplasm, nucleus.
- 其它名称
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
more
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应用
WB: 1:500
ICC: 1:50-1:200
IHC-P: 1:50-1:500
FC: 1:50-1:100
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Fig1: Western blot analysis on 293 cell lysates using anti-Cyclin B1 rabbit polyclonal antibodies.
Fig2: Immunofluorescent staining of F9 cells using anti- Cyclin B1 rabbit polyclonal antibody.
Fig3: Immunofluorescent staining of Hela cells using anti- Cyclin B1 rabbit polyclonal antibody.
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