Anti-UBE1C antibody

Anti-UBE1C antibody

• 概述

• 产品描述Ubiquitin is an abundant, highly conserved protein found in all eukaryotic cells either free or covalently attached to cellular proteins. The primary function of ubiquitin in mammalian systems is to clear abnormal, foreign and improperly folded proteins by targeting them for proteosome degradation. UBA3 (ubiquitin-like modifier activating enzyme 3), also known as NEDD8-activating enzyme E1 catalytic subunit or UBE1C (ubiquitin-activating enzyme E1C), is a 463 amino acid protein belonging to the ubiquitin-activating E1 family and UBA3 subfamily. Ubiquitously expressed, UBA3 acts as an activator to NEDD8, a ubiquitin-like protein, thus regulating cell division, signaling and embryogenesis. UBA3 exists as two isoforms due to alternative splicing events.
• 产品名称Anti-UBE1C antibody
• 分子量52 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型兔多抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃after thawing. Aliquot store at -20℃or -80℃Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus. Cytoplasm.
• 其它名称
  • DKFZp566J164 antibody
  • EC 6.3.2. antibody
  • hUba3 antibody
  more

• 应用

  WB: 1:500
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of UBE1C on human liver tissue lysate using anti-UBE1C antibody at 1/200 dilution.

   

  

  Fig2: ICC staining UBE1C in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining UBE1C in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining UBE1C in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-UBE1C antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-UBE1C antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UBE1C antibody. Counter stained with hematoxylin.

   

  

  Fig8: Flow cytometric analysis of HUVEC cells with UBE1C antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！