Anti-IREB2 antibody
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概述
- 产品描述Iron metabolism is essential for sustaining mammalian homeostasis. Iron uptake and distribution is a highly regulated process in mammalian cells that is monitored by two iron sensing proteins: IRP-1 and -2 (iron regulatory protein-1 and -2), also known as iron responsive element-binding protein-1 and -2 (IRE-BP-1 and -2) or aconitase 1 and 2. IRP-1 and IRP-2 are important soluble regulatory factors that mediate iron uptake and storage in mammalian cells. They are capable of either repressing translation or enhancing mRNA stability by associating with stem-loop motifs known as iron-responsive elements (IREs). IRPs respond to stress mediators, iron concentration and signaling factors, including nitrogen monoxide, cytokines and hydrogen peroxide.
- 产品名称Anti-IREB2 antibody
- 分子量105/37 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃after thawing. Aliquot store at -20℃or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein A purified.
- 亚细胞定位Cytoplasm.
- 其它名称
- ACO3 antibody
- FLJ23381 antibody
- IRE BP2 antibody
more
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应用
WB: 1:500-1:1000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of IREB2 on different lysates using anti-IREB2 antibody at 1/500 dilution.
Positive control:
Lane 1: K562
Lane 2: Mouse kidney tissue Lane 3: Rat skin tissue
Fig2: ICC staining IREB2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining IREB2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining IREB2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IREB2 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IREB2 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of K562 cells with IREB2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black)
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
