Anti-Smoothelin antibody
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概述
- 产品描述Structural protein of the cytoskeleton. This gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
- 产品名称Anti-Smoothelin antibody
- 分子量99 kDa
- 种属反应性Human
- 验证应用WB,IHC
- 抗体类型兔多抗
- 免疫原Recombinant protein within human Smoothelin aa 300-487.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Cytoplasm, Cytoskeleton.
- 其它名称
- FLJ35168 antibody
- FLJ35365 antibody
- FLJ35620 antibody
more
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应用
WB: 1:500-1:1,000
IHC: 1:50
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Fig1: Western blot analysis of Smoothelin on PC-3M cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Immunohistochemical analysis of paraffin-embedded human uterine fibroids tissue using anti-Smoothelin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 mins. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody ) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
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