Anti-SHP-1(PTPN6) antibody
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概述
- 产品描述The steady state of protein tyrosyl phosphorylation in cells is regulated by the opposing action of tyrosine kinases and protein tyrosine phosphatases (PTPs). Several groups have independently identified a non-transmembrane PTP, designated SH-PTP1 (also known as PTP1C, HCP and SHP), which is primarily expressed in hematopoietic cells and characterized by the presence of two SH2 domains N-terminal to the PTP domain. SH2 domains generally mediate the association of regulatory molecules with specific phosphotyrosine-containing sites on autophosphorylated receptors, thereby controlling the initial interaction of receptors with these substrates. A second and much more widely expressed PTP with SH2 domains, SH-PTP2 (also designated PTP1D and Syp), has been identified. Strong sequence similarity between SH-PTP2 and the Drosophila gene corkscrew (CSW) and their similar patterns of expression suggest that SH-PTP2 is the human corkscrew homolog.
- 产品名称Anti-SHP-1(PTPN6) antibody
- 分子量68 kDa
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种属反应性Human,Mouse,Rat
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验证应用WB,ICC,IHC-P,FC
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抗体类型兔多抗
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免疫原Recombinant protein
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偶联Non-conjugated
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性能
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形态Liquid
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浓度1 mg/mL.
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存放说明Store at +4℃ after thawing. Aliquot store at -20℃or -80℃. Avoid repeated freeze / thaw cycles.
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存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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亚型IgG
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纯化方式Protein A purified.
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亚细胞定位Cytoplasm. Nucleus.
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其它名称
more
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70Z-SHP antibody
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EC 3.1.3.48 antibody
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HCP antibody
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应用
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:200
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Fig1: Western blot analysis of SHP1 on mouse spleen tissue lysate using anti- SHP1 antibody at 1/5,000 dilution.
Fig2: ICC staining SHP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining SHP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- SHP1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti- SHP1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti- SHP1 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of Jurkat cells with SHP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
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