Anti-Dux antibody

Anti-Dux antibody

• 概述

• 产品描述The double homeobox (DUX) proteins are encoded by 3.3-kilobase repeats found throughout the human genome. The DUX family includes DUX1, DUX2, DUX3, DUX4 and DUX5. Each of these family members, excluding DUX2, contains two homeobox domains. DUX2 contains only one homeobox domain. DUX1 and DUX5 are identical to one another and they share 98% identity with DUX3 and approximately 70% identity with DUX2. The genes encoding DUX5 and DUX3 both contain additional start sites that result in N-terminal extended isoforms. The homeodomains found in DUX5 and DUX1 are similar to those found in Pax-3, Pax-7, OTX1 and OTX2. DUX4, also known as DUX10, is capable of forming homodimers. In addition, the gene encoding DUX4 maps within the D4Z4 repeat unit that has been implicated in Facioscapulohumeral muscular dystrophy (FSHD).
• 产品名称Anti-Dux antibody
• 分子量74 kDa
• 种属反应性Mouse
• 验证应用WB,ICC
• 抗体类型兔多抗
• 免疫原Synthetic peptide within mouse Dux aa 520-570.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/ml.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Peptide affinity purified.
• 亚细胞定位Nucleus.
  
• 其它名称
  • Double homeobox 4 antibody
  • Double homeobox protein 10 antibody
  • Double homeobox protein 4 antibody
  more

• 应用

  WB: 1:500
  ICC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of Dux on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibodwas used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: Non-transfected 293T cells lysate
  Lane 2: His-tagged Dux transfected 293T cells lysate

   

  

  Fig2: ICC staining of Dux in Non-transfected 293T and His-tagged Dux transfected 293T cells cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody  for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

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