Anti-AP-4 complex subunit sigma antibody

Anti-AP-4 complex subunit sigma antibody

• 概述

• 产品描述Subunit of novel type of clathrin- or non-clathrin-associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system.
• 产品名称Anti-AP-4 complex subunit sigma antibody
• 分子量17 kDa
• 种属反应性Arabidopsis thaliana
• 验证应用IF,IHC-P,ELISA
• 抗体类型兔多抗
• 免疫原Synthetic peptide within A. thaliana AP-4 complex subunit sigma aa 50-100.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Peptide affinity purified.
• 亚细胞定位Trans-Golgi network, coated pit.
• 其它名称
  • AP-4 complex subunit sigma
  • AP-4 adaptor complex subunit sigma
  • Adaptor-related protein complex 4 subunit sigma
  more

• 应用

  IF:1:50-1:100
  IHC-P:1:50-1:200
  ELISA:1:10,000-1:20,000

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  Fig1: Immunofluorescence staining of paraffin- embedded A. thaliana using anti-Rubisco activase rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed withat 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-AP-4 complex subunit sigma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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