Anti-Claudin 18.1 antibody

Anti-Claudin 18.1 antibody

• 概述

• 产品描述This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified.
• 产品名称Anti-Claudin 18.1 antibody
• 分子量Predicted band size 28 kDa.
• 种属反应性Human,Mouse
• 验证应用WB,FC
• 抗体类型兔多抗
• 免疫原Synthetic peptide within human Claudin18 aa 28-80.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Peptide affinity purified
• 亚细胞定位Cell membrane.
• 其它名称
  • Claudin 18.1 antibody
  • Claudin-18.1 antibody
  • CLD18.1_HUMAN antibody
  more

• 应用

  WB:1:500-1:1,000
  FC:1:50-1:100

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  Fig1: Western blot analysis of Claudin18 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: human stomach tissue lysate
  Lane 2: AGS cell lysate

   

  

  Fig2: Flow cytometric analysis of Claudin18 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody ((red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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