Anti-HDG11 antibody
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概述
- 产品描述Transcription factor which acts as positive regulator of drought stress tolerance. Can transactivate CIPK3, NCED3 and ERECTA. Transactivates several cell-wall-loosening protein genes by directly binding to HD motifs in their promoters. These target genes play important roles in coordinating cell-wall extensibility with root development and growth. Transactivates CYP74A/AOS, AOC3, OPR3 and 4CLL5/OPCL1 genes by directly binding to HD motifs in their promoters. These target genes are involved in jasmonate (JA) biosynthesis, and JA signaling affects root architecture by activating auxin signaling, which promotes lateral root formation. Acts as negative regulator of trichome branching. Required for the establishment of giant cell identity on the abaxial side of sepals. May regulate cell differentiation and proliferation during root and shoot meristem development.
- 产品名称Anti-HDG11 antibody
- 分子量79 kDa
- 种属反应性Arabidopsis thaliana
- 验证应用WB,IHC-P,ELISA
- 抗体类型兔多抗
- 免疫原Synthetic peptide within A. thaliana HDG11 aa 620-690.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Nucleus.
- 其它名称
- Homeobox-leucine zipper protein HDG11
- HD-ZIP protein HDG11
- Homeodomain GLABRA 2-like protein 11
more
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应用
WB:1:500-1:2,000
IHC-P:1:50-1:200
ELISA:1:250-1:256,000
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Fig1: Western blot analysis of HDG11 on HDG11 recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HDG11 recombinant protein with primary antibody at 1:200 dilutions
Lane 2: HDG11 recombinant protein with primary antibody at 1:1,000 dilutions
Fig2: Immunohistochemical analysis of paraffin-embedded A. thaliana
tissue using anti-HDG11 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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