Anti-MCL1 antibody

Anti-MCL1 antibody

• 概述

• 产品描述B-cell CLL/lymphoma 2 (Bcl-2) blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Myeloid cell leukemia 1 (Mcl-1) shares sequence homology with Bcl-2 and further resembles Bcl-2 in that its expression promotes cell viability. p53 and Mcl-1 demonstrate opposing effects on mitochondrial apoptosis by mediating Bcl-2 antagonist killer (Bak) activity. Mcl-1 is an important and specific regulator that is necessary for the homeostasis of early hematopoietic progenitors. Glycogen synthase kinase 3 (GSK3) controls Mcl-1 stability, which has an effect on the regulation of apoptosis by growth factors, PI 3-kinase and AKT. Mice with a deficiency of the Mcl-1 protein show a significant reduction in B and T lymphocytes similar to the effects observed in IL-7- or IL-7R-deficient mice.

• 产品名称Anti-MCL1 antibody

• 分子量37 / 29 kDa

• 种属反应性Human,Mouse

• 验证应用ICC,IHC-P

• 抗体类型兔多抗

• 免疫原Peptide

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Membrane. Cytoplasm. Mitochondrion.

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• ​其它名称

  more

  • Bcl 2 related protein EAT/mcl1 antibody

  • Bcl-2-like protein 3 antibody

  • Bcl-2-related protein EAT/mcl1 antibody

• 应用

  ICC: 1:50-1:200
  IHC-P: 1:50-1:200

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  Fig1: ICC staining MCL1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig2: ICC staining MCL1 in SK-BR-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining MCL1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MCL1 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Thioredoxin antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Thioredoxin antibody. Counter stained with hematoxylin.

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