Anti-CDK2 antibody

Anti-CDK2 antibody

• 概述

• 产品描述Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. It acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1.
• 产品名称Anti-CDK2 antibody
• 分子量~33kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型兔多抗
• 免疫原Synthetic peptide within C terminal residues of human CDK2.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Peptide affinity purified
• 亚细胞定位Cytoplasm. Nucleus
• 其它名称
  • Cdc2 related protein kinase antibody
  • cdc2-related protein kinase antibody
  • CDC28 antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:50-1:200
  IHC-P: 1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of CDK2 on different cell lysates using anti-CDK2 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: A549
  Lane 2: F9
  Lane 3: PC-12
  Lane 4: Mouse thymus
  Lane 5: Human liver

   

  

  Fig2: ICC staining CDK2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CDK2 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CDK2 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of Hela cells with CDK2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

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