Anti-STIM1 antibody
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概述
- 产品描述Ca2+ influx is essential for a variety of cellular functions, including secretion and transcription. Stromal interaction molecule 1 (Stim1) is a ubiquitously expressed cell surface transmembrane glycoprotein that plays a role in mediating Ca2+ influx following the depletion of intracellular Ca2+ stores. Stim1 functions in the endoplasmic reticulum (ER) where it acts as a Ca2+ sensor via its EF-hand domain, causing large conformational changes. When Ca2+ levels drop, Stim1 translocates from the ER to the plasma membrane, where it activates the calcium release-activated calcium (CRAC) channel subunit, TMEM142A/Orai1. Stim2 is a potent inhibitor of Stim1-mediated, store-operated calcium (SOC) entry. Stim1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions.
- 产品名称Anti-STIM1 antibody
- 分子量86kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IP,IF,IHC-P
- 抗体类型兔多抗
- 免疫原Amino acids 441-620 mapping near the C-terminus of Stim1 of human origin.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified
- 亚细胞定位Cytoplasm, Endoplasmic reticulum membrane Cell membrane
- 其它名称
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- GOK antibody
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应用
WB: 1:100-1:1,000
IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
IF: 1:50-1:500
IHC-P: 1:50-1:500
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Fig1: Western blot analysis of Stim1 expression in non-transfected 293T (A), human Stim1 transfected 293T (B) and A-10 (C) whole cell lysates.
Fig2: Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic and membrane staining of Purkinje cells and cytoplasmic staining of cells in granular layer and cells in molecular layer.
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