Anti-CD3 epsilon antibody

Anti-CD3 epsilon antibody

• 概述

• 产品描述CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) These chains associate with the T cell receptor (TCR) and the ζ-chain to generate an activation signal in T lymphocytes.
• 产品名称买二赠二Anti-CD3 epsilon antibody
• 分子量23 kDa
• 种属反应性Human,Mouse
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型兔多抗
• 免疫原This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to N-terminal residues of CD3 epsilon.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Peptide affinity purified
• 亚细胞定位Membrane.
• 其它名称
  • CD3 epsilon antibody
  • CD3e antibody
  • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
  more

• 应用

  WB: 1:1,000
  ICC: 1:200
  IHC-P: 1:200
  FC: 1:100-1:200

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  Fig1: Western blot analysis of CD3E on different cell lysates using anti-CD3E antibody at 1/1000 dilution.

   

  

  Fig2: ICC staining CD3E in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining CD3E in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD3E antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD3E antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD3E antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Jurkat cells with CD3E antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

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