Anti-PKC antibody

Anti-PKC  antibody

• 概述

• 产品描述Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2
• 产品名称Anti-PKC antibody
• 分子量67-83kD(α 76/δ/β 77/γ 78/ θ/ε83 / ζ 67） kDa
• 种属反应性Human, Mouse, Rat
• 验证应用WB, IHC-P, IF
• 抗体类型兔多抗
• 免疫原The antiserum was produced against synthesized peptide derived from human PKC. AA range:623-672
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• 亚型IgG
• 纯化方式Immunogen affinity purified.
• 其它名称
  • PRKCA antibody; PKCA antibody; PRKACA antibody; Protein kinase C alpha type antibody; PKC-A antibody; PKC-alpha antibody; PRKCB antibody; PKCB antibody; PRKCB1 antibody; Protein kinase C beta type antibody; PKC-B antibody; PKC-beta antibody; PRKCD antibody; Protein kinase C delta type antibody; Tyrosine-protein kinase PRKCD antibody; nPKC-delta antibody; PRKCE antibody; PKCE antibody; Protein kinase antibody;

• 应用

  WB: 1:500-1:2000
  IHC-P: 1:100-1:300
  IF: 1:100-1:500

•  

  

  Fig1: Immunofluorescence analysis of human-liver tissue. 1,PKC Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,PKC Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

   

  

  Fig3: Western Blot analysis of various cells using PKC Polyclonal Antibody diluted at 1：2000

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