Anti-COP1 antibody
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概述
- 产品描述domain:The RING finger domain, in addition to its role in ubiquitination, functions as a structural scaffold to bring two clusters of positive-charged residues within spatial proximity to mimic a bipartite nuclear localization signal (NLS). E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. induction:By p53/TP53. pathway:Protein modification; protein ubiquitination. Belongs to the COP1 family. Contains 1 RING-type zinc finger. Contains 7 WD repeats. subcellular location:In the nucleus, it forms nuclear speckles. subunit:Homodimer. Homodimerization is mediated by the coiled coil domain. Component of the DCX DET1-COP1 ubiquitin ligase complex at least composed of RBX1, DET1, DDB1, CUL4A and COP1. Isoform 2 does not interact with CUL4A but still binds to RBX1, suggesting that the interaction may be mediated by another culllin protein. Isoform 1 and isoform 2 interact with CUL5 but not with CUL1, CUL2 not CUL3. Interacts with bZIP transcription factors JUN, JUNB and JUND but not with FOS, ATF2 nor XBP1. Interacts with p53 (TP53). tissue specificity:Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.
- 产品名称Anti-COP1 antibody
- 分子量80 kDa
- 种属反应性Human,Mouse
- 验证应用WB
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from human RFWD2. AA range:661-710
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified.
- 其它名称RFWD2 antibody; COP1 antibody; RNF200 antibody; E3 ubiquitin-protein ligase RFWD2 antibody; Constitutive photomorphogenesis protein 1 homolog antibody; hCOP1 antibody; RING finger and WD repeat domain protein 2 antibody; RING finger protein 200 antibody;
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