Anti-Dbf4 antibody
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概述
- 产品描述Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle. induction:Induced in G1 phase at low level, increased during G1-S phase and remain high during S and G2-M phase. PTM:Phosphorylated upon DNA damage, probably by ATM or ATR. Contains 1 DBF4-type zinc finger. Contains 2 BRCT domains. subunit:Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2L, ORC4L and ORC6L. tissue specificity:Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
- 产品名称Anti-Dbf4 antibody
- 分子量77 kDa
- 种属反应性Human, Mouse, Monkey
- 验证应用WB, IHC-P
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from human DBF4. AA range:10-59
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified.
- 其它名称DBF4 antibody; ASK antibody; DBF4A antibody; ZDBF1 antibody; Protein DBF4 homolog A antibody; Activator of S phase kinase antibody; Chiffon homolog A antibody; DBF4-type zinc finger-containing protein 1 antibody;
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应用
WB: 1:500-1:2000
IHC-P: 1:100-1:300
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Fig1: Western Blot analysis of various cells using Dbf4 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Fig2: Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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