Anti-CA VB antibody
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概述
- 产品描述Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles.
- 产品名称Anti-CA VB antibody
- 分子量38 kDa
- 种属反应性Human, Mouse, Rat
- 验证应用WB, IHC-P, IF
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from human CA5B. AA range:241-290
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified.
- 其它名称CA5B antibody; Carbonic anhydrase 5B antibody; mitochondrial antibody; Carbonate dehydratase VB antibody; Carbonic anhydrase VB antibody; CA-VB antibody;
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应用
WB: 1:500-1:2000
IHC-P: 1:100-1:300
IF: 1:100-1:500
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Fig1: Western Blot analysis of 3T3 cells using CA VB Polyclonal Antibody
Fig2: Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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